Review



mpg v6 3  (Azenta)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Azenta mpg v6 3
    Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG <t>v6.3,</t> derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).
    Mpg V6 3, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mpg v6 3/product/Azenta
    Average 86 stars, based on 1 article reviews
    mpg v6 3 - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine"

    Article Title: Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf1423

    Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG v6.3, derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).
    Figure Legend Snippet: Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG v6.3, derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).

    Techniques Used: Sequencing, Derivative Assay, Construct, Transfection



    Similar Products

    mpg v6 3  (Azenta)
    86
    Azenta mpg v6 3
    Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG <t>v6.3,</t> derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).
    Mpg V6 3, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mpg v6 3/product/Azenta
    Average 86 stars, based on 1 article reviews
    mpg v6 3 - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG v6.3, derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).

    Journal: Nucleic Acids Research

    Article Title: Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine

    doi: 10.1093/nar/gkaf1423

    Figure Lengend Snippet: Design and screening of triple base editors (ACG-BEs). ( a ) The process of ACG-BEs catalyzes three types of base substrates (A, C, and G) to generate DNA sequence diversity. Adenosine deaminase refers to evolved Escherichia coli adenosine deaminase mutants (TadA-8e, T AD AC-3.1, T AD AC-3.155, and TadA-dual); nCas9, Cas9 D10A; MPG v6.3, derived from evolved N-methylpurine DNA glycosylase. ( b ) Schematics of the constructs for ACG-BEs. bNLS, bipartite nuclear localization signals; T AD AC-3.1, T AD AC-3.155, and TadA-dual, derived from evolved E. coli adenosine deaminase; nCas9, Cas9 D10A; MPG v3, and MPG v6.3, derived from evolved N-methylpurine DNA glycosylase; linkers are also shown. ( c ) Base-editing outcomes of ACG-BEs at the endogenous target PLS3-AS1 -sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments). ( d ) Allele table for PLS3-AS1 -sg1 in HEK293T cells after ACG-BE7 transfection. The target site allele is boxed in a gray line. The percentile and sequencing read of each allele at one representative of three independent experiments are listed on the right. ( e ) The allele base editing efficiency of ACG-BEs across the protospacer at the endogenous target PLS3-AS1- sg1 in HEK293T cells. Data are means ± SD ( n = 3 independent experiments).

    Article Snippet: Human codon-optimized TadA-dual, T AD A-3.1, T AD A-3.155, MPGv3, and MPG v6.3 were synthesized by Genewiz (Suzhou, China).

    Techniques: Sequencing, Derivative Assay, Construct, Transfection